Head office :
Centre INRA - Tours
Domaine de l’Orfrasière
37380 Nouzilly, France

Phone :
+33 (0) 2 47 42 79 35

Email :
contact@repropharm.com

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LH DETECT® for ovines

LH DETECT® for ovines

An immuno-enzymatic (not radio-active) assay kit to accurately measure Luteinizing Hormone in various animal species.

  • LH DETECT® can be used in a laboratory for highly accurate measurements and detection of the pre-ovulatory LH surge, from a blood, serum or plasma sample. For sheep and goats, measurement can also be done on a milk sample.
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Description

LH DETECT® is particularly useful for the following works :

  • Following the LH secretions during the male and female sexual cycle
  • Studying the micropulsatility of LH secretions
  • Determining the most appropriate time to proceed to an artificial insemination insofar as the time between LH surge and ovulation is known and constant for each specie
  • Any other study requiring an accurate LH measurement

LH detection can be done from blood, plasma or serum samples, and even in the milk of sheeps.

Choose your substrate: ABTS or TMB (recommanded for quantitative works); and your kind of plate: entire one or  strip one (divisible in 16-well segments).

LH DETECT® is build under an exclusive and wordlwide INRA licence.

 
Principle

LH DETECT® is an ELISA sandwich type immunoenzymatic test. The enzymatic activity is revealed by a chromogenic substrate.

Reaction intensity is proportionnal to LH concentration in the test sample. They evaluation may be performed visually (qualitative) or measured with a spectrophotometer.

For qualitative assay, a positive control sample permits the reaction validation. For this use, we recommend using ABTS as substrate.

For quantitative assay, a scale of standards, produced with the LH of the concerning specie, is used. In this case, we recommend using TMB as substrate.

You can choose between two types of plate: entire one, or strip one divisible in 16-well segments. The strip one allows avoiding defrosting all the wells when only a few samples are going to be tested.
 

 

 
Procedure

Please find below the schematic procedure of LH DETECT®. It describes all the procedure, including sample and reagents preparation.

More detailed data are sent with any product order through our website. For any question regarding to the use of LH DETECT®, please ask to our technical team by using the 'Contact' email.

Download the schematic procedure

 

 

 
Applications

The time interval between LH surge and ovulation is constant and estimated at 24h ± 2h among ewe (Cumming IA et al, 1973; Biol Reprod. 9, 24-29). On the contrary, the interval between œstrus beginning and ovulation is very variable and doesn't bring such accuracy.

Among females being in lactation, LH DETECT® also permits to measure LH in milk. The LH surge duration is longer in milk than in blood, wich permits to space samplings out. Of course, LH concentrations are smaller.

  • Study of LH secretions micropulsatility :
    Blood samplings are done every 20 minutes during several hours. We recommend to group the samples for each animal in order to be able to measure them on the same plate.
  • Preovulatory LH surge detection on synchronized œstrus :
    The blood samplings can be done every 4 hours, from 24 hours after the end of the progestational-eCG treatment to 55 hours (time of the systematic artificial insemination).
  • Detection in milk :
    Samplings in milk have to be realised every 8 or 12 hours, at the morning and evening milking times, during the same period than blood samplings (24 to 55 hours after sponge withdrawal).

The correlation coefficient obtained with a reference RIA measurement (Pelletier J. et al, 1982; J. Reprod. Fertil.,64, 341-346) is 0.98.

 

 

 
Publications

LH-DETECT® in Biology of Reproduction, 1999 / 61, 209 - 218

ROY F, COMBES B, VAIMAN D, CRIBIU EP, POBEL T, DELETANG F, COMBARNOUS Y, GUILLOU F, MAUREL MC 1999 Humoral Immune Response to Equine Chorionic Gonadotropin in Ewes: Association with Major Histocompatibility Complex and Interference with Subsequent Fertility. Biology of Reproduction 61:209-218

LH-DETECT® in Animal Reproduction Science, 2009

BEN SALEM I, REKIK M, GONZALES-BULNES A, LASSOUED N, KRAIEM K 2009 Differences in preovulatory follicle dynamics and timing of preovulatory LH surge affect fertility of maiden sheeps reared in semi-arid extensive conditions. Animal Reproduction Science 117(1-2):60-66

LH-DETECT® in Toxicological Sciences, 2010 / 117 (1): 54-62
SEVERINE H COLLET, NICOLE PICARD-HAGEN, CATHERINE VIGUIÉ, MARLENE Z LACROIX, PIERRE-LOUIS TOUTAIN et VERONIQUE GAYRARD 2010 Estrogenicity of Bisphenol A: A Concentration-Effect Relationship on Luteinizing Hormone Secretion in a Sensitive Model of Prepubertal Lamb. Toxicol. Sci. (2010) 117 (1): 54-62

LH-DETECT® in Theriogenology, 2009 / 71(6) : 1018 - 1025
I CONTRERAS SOLIS, B VASQUEZ, T DIAZ, C LETELIER, A LOPEZ-SEBASTIEN et A GONZALES-BULNES 2009 Efficiency of estrous synchronization in tropical sheep by combining short-interval cloprostenol-based protocols and "male effect". Theriogenology 71 (6): 1018 - 1025

LH-DETECT® in Animal Products from the Mediterranean Area, 2006 / 119: 343-347
M.I. VASQUES, S. CAVACO-GONÇALVES, C.C. MARQUES, J.P. BARBAS, M.C. BAPTISTA, T.P. CUNHA & A.E.M. HORTA 2006 The effect of ram exposure previous to progestagen oestrus synchronization on corpus luteum function and fertility in crossbred ewes. Animal Products from the Mediterranean Area, 2006 / 119: 343-347

LH-DETECT® in Journal of Animal Reproduction Science, 2009 / 114 (4), 384-392
IGNACIO CONTRERAS-SOLIS, B. VASQUEZ, T. DIAZ, C. LETELIER, A. LOPEZ-SEBASTIAN, A. GONZALEZ-BULNES 2009 Ovarian and endocrine responses in tropical sheep treated with reduced doses of cloprostenol. Journal of Animal Reproduction Science (2009) 114 (4): 384-392

LH-DETECT® in Theriogenology, 2006 / 65 (7) 1265–1278
R. BONI, R. SANTILLO, G. MACCHIA, P. SPINELLI, G. FERRANDINO, A. D'ANIELLO 2006 d-Aspartate and reproductive activity in sheep. Theriogenology, 2006 / 65 (7) 1265-1278

 

 

 
 

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