Head office :
Centre INRA - Tours
Domaine de l’Orfrasière
37380 Nouzilly, France

Phone :
+33 (0) 2 47 42 79 35

Email :
contact@repropharm.com

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LH DETECT® for porcines

LH DETECT® for porcines

An immuno-enzymatic (not radio-active) assay kit to accurately measure Luteinizing Hormone in various animal species.

  • LH DETECT® can be used in a laboratory for highly accurate measurements and detection of the pre-ovulatory LH surge, from a blood, serum or plasma sample. For sheep and goats, measurement can also be done on a milk sample.
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Description

LH DETECT® is particularly useful for the following works :

  • Following the LH secretions during the male and female sexual cycle
  • Studying the micropulsatility of LH secretions
  • Determining the most appropriate time to proceed to an artificial insemination insofar as the time between LH surge and ovulation is known and constant for each specie
  • Any other study requiring an accurate LH measurement

LH detection can be done from blood, plasma or serum samples, and even in the milk of sheeps.

Choose your substrate: ABTS or TMB (recommanded for quantitative works); and your kind of plate: entire one or  strip one (divisible in 16-well segments).

LH DETECT® is build under an exclusive and wordlwide INRA licence.

 
Principle

LH DETECT® is an ELISA sandwich type immunoenzymatic test. The enzymatic activity is revealed by a chromogenic substrate.

Reaction intensity is proportionnal to LH concentration in the test sample. They evaluation may be performed visually (qualitative) or measured with a spectrophotometer.

For qualitative assay, a positive control sample permits the reaction validation. For this use, we recommend using ABTS as substrate.

For quantitative assay, a scale of standards, produced with the LH of the concerning specie, is used. In this case, we recommend using TMB as substrate.

You can choose between two types of plate: entire one, or strip one divisible in 16-well segments. The strip one allows avoiding defrosting all the wells when only a few samples are going to be tested.

 

 
Procedure

Please find below the schematic procedure of LH DETECT®. It describes all the procedure, including sample and reagents preparation.

More detailed data are sent with any product order through our website. For any question regarding to the use of LH DETECT®, please ask to our technical team by using the 'Contact' email.

Download the schematic procedure

 

 

 
Applications

The blood samplings rate is 2 times for each 24 hours. For exemple, at 8am and 16pm. Samplings can begin the 4th day after the end of the synchronization treatment, and stop 24 hours after the end of the oestrus comportment.

 

 
Publications

LH-DETECT® in Reproduction, Nutrition and Development, 2000 / 40, 1 -12

TERQUI M, GUILLOUET P, MAUREL MC, MARTINAT-BOTTE F 2000 Relationship between peri-oestrus progesterone levels and time of ovulation by echography in pigs and influence of the interval between ovulation and artificial insemination (I.A.) on litter size. Reproduction & Development 40:1-12

 

 
 

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