Head office :
Centre INRA - Tours
Domaine de l’Orfrasière
37380 Nouzilly, France

Phone :
+33 (0) 2 47 42 79 35

Email :
contact@repropharm.com

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LH DETECT® for caprines

LH DETECT® for caprines

An immuno-enzymatic (not radio-active) assay kit to accurately measure Luteinizing Hormone in various animal species.

  • LH DETECT® can be used in a laboratory for highly accurate measurements and detection of the pre-ovulatory LH surge, from a blood, serum or plasma sample. For sheep and goats, measurement can also be done on a milk sample.
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Description

LH DETECT® is particularly useful for the following works :

  • Following the LH secretions during the male and female sexual cycle
  • Studying the micropulsatility of LH secretions
  • Determining the most appropriate time to proceed to an artificial insemination insofar as the time between LH surge and ovulation is known and constant for each specie
  • Any other study requiring an accurate LH measurement

LH detection can be done from blood, plasma or serum samples, and even in the milk of sheeps.

Choose your substrate: ABTS or TMB (recommanded for quantitative works); and your kind of plate: entire one or  strip one (divisible in 16-well segments).

LH DETECT® is build under an exclusive and wordlwide INRA licence.

 
Principle

LH DETECT® is an ELISA sandwich type immunoenzymatic test. The enzymatic activity is revealed by a chromogenic substrate.

Reaction intensity is proportionnal to LH concentration in the test sample. They evaluation may be performed visually (qualitative) or measured with a spectrophotometer.

For qualitative assay, a positive control sample permits the reaction validation. For this use, we recommend using ABTS as substrate.

For quantitative assay, a scale of standards, produced with the LH of the concerning specie, is used. In this case, we recommend using TMB as substrate.

You can choose between two types of plate: entire one, or strip one divisible in 16-well segments. The strip one allows avoiding defrosting all the wells when only a few samples are going to be tested.

 

 

 

 
Procedure

Please find below the schematic procedure of LH DETECT®. It describes all the procedure, including sample and reagents preparation.

More detailed data are sent with any product order through our website. For any question regarding to the use of LH DETECT®, please ask to our technical team by using the 'Contact' email.

Download the schematic procedure

 

 

 
Applications

The time interval between LH surge and ovulation is constant and estimated at 21h ± 3h for 71% of goats (Leboeuf et al . 1996). On the contrary, the time interval "œstrus beginning - ovulation" is pretty variable and so doesn't bring such accuracy.

Like in ovines, LH DETECT® is able to measure LH in milk of females being in lactation. The LH surge duration is longer than in blood, which permits to space samplings out, but LH concentrations are smaller.
 
Here are some exemples :
  • Micropulsatility study :
    Take blood samples every 20 min. We advise to group the samplings for each animal in order to measure them on the same plate.
  • Preovulatory LH surge detection :
    Dans le cas où il n'y a pas détection des chaleurs, les prélèvements sanguins peuvent être réalisés toutes les 4h, de 16h après la fin du traitement progestagène - eCG jusqu'à 43 heures (moment de l'IA systématique).
  • In the case of not detectable heats, blood samplings can be done every 4 hours, from 16 hours after the end of the progestational – eCG treatment, to 43 hours (time of the systematic artificial insemination). In case heats are detected by the aid of a vasectomised goat, samplings begin from the heats appearance and continue every 4 hours for 24 hours. For females not being in œstrus 30 hours after the end of the treatment, blood samplings are done every 4 hours until the artificial insemination (females with belated or nonexistent ovulation).
  • Milk detection :
    Milk samplings are done every 8 or 12 hours, i.e. At the morning and evening milking times, during the same period than blood samplings (from 20 to 43 hours after sponge withdrawal).

The correlation coefficient obtained with a reference RIA measurement (Pelletier et al, 1982, J. Reprod. Fertil., 64, 341-346) is 0.976.

 

 
Publications

LH-DETECT® in Biology of Reproduction, 1999 / 60, 805 - 813

ROY F, MAUREL MC, COMBES B, VAIMAN D, CRIBIU E, LANTIER I, POBEL T, DELETANG F, COMBARNOUS Y, GUILLOU F 1999 The Negative Effect of Repeated Equine Chorionic Gonadotropin Treatment on Subsequent Fertility in Alpine Goats Is Due to a Humoral Immune Response Involving the Major Histocompatibility Complex. Biology of Reproduction 60:805-813

LH-DETECT® in Animal Reproduction Science, 2008

PELLICER-RUBIO MT, LEBOEUF B, BERNELAS D, FORGERIT Y, POUGNARD JL, BONNE JL, SENTY E. BRETON S, BRUN F, CHEMINEAU P 2008 High fertility using artificial insemination during deep anoestrus after induction and synchronisation of ovulatory activity by the "male effect" in lactating goats subjected to treatment with artificial long days and progestagens. Animal Reproduction Science 109(1-4):172-188

 

 
 

 

In the case of not detectable heats, blood samplings can be done every 4 hours, from 16 hours after the end of the progestational – eCG treatment, to 43 hours (time of the systematic artificial insemination). In case heats are detected by the aid of a vasectomised goat, samplings begin from the heats appearance and continue every 4 hours for 24 hours. For females not being in œstrus 30 hours after the end of the treatment, blood samplings are done every 4 hours until the artificial insemination (females with belated or nonexistent ovulation)
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